Super-resolution RESOLFT microscopy of lipid bilayers using a fluorophore-switch dyad. Issue 33 (17th August 2020)

• Record Type: Journal Article
• Authors: Frawley, Andrew T.; Wycisk, Virginia; Xiong, Yaoyao; Galiani, Silvia; Sezgin, Erdinc; Urbančič, Iztok; Vargas Jentzsch, Andreas; Leslie, Kathryn G.; Eggeling, Christian; Anderson, Harry L.
• Is Part Of: Chemical science. Volume 11:Issue 33(2020)
• Page Start: 8955
• Page End: 8960
• Publication Date: 2020-08-17
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d0sc02447c
• Languages: English
• ISSNs: 2041-6520

Abstract

Abstract : A photoswitchable quencher can be used to reversibly turn off the emission from a fluorescent dye, generating a small molecule dyad that is effective for super-resolution RESOLFT microscopy. Abstract : Dyads consisting of a photochromic switch covalently linked to a fluorescent dye allow the emission from the dye to be controlled by reversible photoisomerization of the switch; one form of the switch quenches fluorescence by accepting energy from the dye. Here we investigate the use of dyads of this type for super-resolution imaging of lipid bilayers. Giant unilamellar vesicles stained with the dyads were imaged with about a two-fold resolution-enhancement compared with conventional confocal microscopy. This was achieved by exciting the fluorophore at 594 nm, using a switch activated by violet and red light (405/640 nm).