Acute kidney injury caused by the intraperitoneal injection of Bothrops jararaca venom in rats. Issue 17 (1st September 2020)

• Record Type: Journal Article
• Authors: Yoshida, Edson Hideaki; Dini, Murilo Melo Juste; Campanholi, Jessica; Cogo, José Carlos; Grotto, Denise; Hyslop, Stephen; Hanai-Yoshida, Valquíria Miwa; Oshima-Franco, Yoko
• Is Part Of: Natural product research. Volume 34:Issue 17(2020)
• Page Start: 2533
• Page End: 2538
• Publication Date: 2020-09-01
• Subjects: Acute kidney injury -- blood incoagulability -- Bothrops jararaca; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2018.1543675
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: We examined the ability of Bothrops jararaca venom (12.5 mg/kg) injected intraperitoneally (i.p.) to cause acute kidney injury (AKI) in rats. Blood urea and creatinine (AKI biomarkers, in g dL −1 ) were elevated after 2 h in venom-treated rats (urea: from 0.41 ± 0.1 to 0.7 ± 0.03; creatinine from 46.7 ± 3.1 to 85 ± 6.7; p < 0.05; n = 3 each), with no change in circulating reduced glutathione. Venom-treated rats survived for ∼6 h, at which point platelets were reduced (×10 3 µL −1 ; from 763.8 ± 30.2 to 52.5 ± 18.2) whereas leukocytes and erythrocytes were slightly increased (from 4.7 ± 0.3 to 6.6 ± 0.1 × 10 3 µL −1 and from 8.38 ± 0.1 to 9.2 ± 0.09 × 10 6 µL −1, respectively; p < 0.05); blood protein (5.2 ± 0.4 g dL −1 ) and albumin (2.7 ± 0.1 g dL −1 ) were normal, whereas blood and urinary urea and creatinine were increased. All parameters returned to normal with antivenom given 2 h post-envenomation. The i.p. injection of venom caused AKI similar to that seen with other routes of administration. Graphical Abstract: