Antioxidant activity optimization and GC-MS profile of aqueous extracts of Vernonanthura patens (Kunth) H. Rob. leaves. Issue 17 (1st September 2020)

• Record Type: Journal Article
• Authors: Chóez-Guaranda, Ivan; Ruíz-Barzola, Omar; Ruales, Jenny; Manzano, Patricia
• Is Part Of: Natural product research. Volume 34:Issue 17(2020)
• Page Start: 2505
• Page End: 2509
• Publication Date: 2020-09-01
• Subjects: ABTS -- catechin -- DPPH -- gallic acid -- RSM -- TEAC; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2018.1539978
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: The temperature and extraction time of aqueous extracts of Vernonanthura patens (Kunth) H. Rob. (AEVP) leaves obtained by decoction were optimized for maximum recovery of DPPH radical scavenging activity, ABTS + inhibition activity, total phenolic content (TPC) and total flavonoids content (TFC) using response surface methodology (RSM). A central composite design (CCD) of 13 experimental runs was applied and second order polynomial models were used to describe the responses of the assessed extraction parameters. The optimized conditions: 79.79 °C and 126.23 minutes were found using the composite desirability function. The scavenging activity of assessed extracts could be correlated mostly to the presence of malic acid, succinic acid, α-ketoglutaric acid, citric acid, m-hydroxybenzoic acid, caffeic acid, inositol, and β-amyrin detected by gas chromatography-mass spectrometry (GC-MS). These results have not been reported and support the potential application of AEVP as natural source of antioxidants. Graphical Abstract: