Baculovirus transit through insect cell membranes: A mechanistic approach. (21st September 2020) - Zhang, Youhong
Highlights: A novel mechanistic model of the early stages of viral infection. Excellent fit to experimental evidence. The maximum number of virions that Sf9 cells can carry: 55 viruses/cell, is reported. Cells that carry virions on their surface, in their interior, or both are distinguished. Analytical mathematical solution renders satisfactory results. Abstract: Baculovirus systems are used for various purposes, but the kinetics of the infection process is not fully understood yet. We investigated the dynamics of virion movement from a medium toward the interior of insect cells and established a mechanistic model that shows an excellent fit to experimental results. It also makes possible a description of the viral dynamics on the cell surface. A novel measurement method was used to distinguish between infected cells that carry virions on their surfaces, cells that carry virions in their interior, and those carrying virions both inside and on their surface. The maximum number of virions carried by a cell: 55 viruses/cell, and the time required for viral internalization, 0.8 h, are reported. This information is particularly useful for assessing the infection efficacy and the required number of virions needed to infect a given cell population. Although our model specifically concerns the infection process of Sf9 insect cells by baculovirus, it describes general features of viral infection. Some of the model features may eventually be applicable in the studies towards palliation of the COVID-19 outbreak.