Beta-1, 6 glucan converts tumor-associated macrophages into an M1-like phenotype. (1st November 2020)

• Record Type: Journal Article
• Authors: Cheng, Hairong; Sun, Lin; Shen, Danyang; Ren, Ai; Ma, Fangli; Tai, Guihua; Fan, Luodi; Zhou, Yifa
• Is Part Of: Carbohydrate polymers. Volume 247(2020)
• Publication Date: 2020-11-01
• Subjects: β-1, 6-Glucan -- Amillariella Mellea and Lentinus edodes -- Macrophages polarization -- TLR2 -- Anti-tumor; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.116715
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: A β-D-(1→6) glucan converts M2-like macrophages to M1-like phenotype. The converting effect is mediated by Akt/NF-κB and MAPK via TLR2 receptor. β-1, 6-glucan inhibits colon cancer cell growth via its macrophage converting effect. Abstract: Tumor-associated macrophages (TAMs) with an M2-like phenotype have been linked to immunosuppression and resistance to chemotherapies of cancer, thus targeting TAMs has been an attractive therapeutic strategy to cancer immunotherapy. We have reported that the β-D-(1→6) glucan (AAMP-A70) isolated from Amillariella Mellea could promote macrophage activation. The present study showed that the β-1, 6-glucan could promote the transformation of M2-like macrophages to M1-like phenotype and inhibit the viability of colon cancer cells in vitro and in vivo . On a cellular mechanistic level, the β-1, 6-glucan reset tumor-promoting M2-like macrophages to tumor-inhibiting M1-like phenotype via increasing the phosphorylation of Akt/NF-κB and MAPK. Further, TLR2 was identified as the receptor of β-1, 6-glucan in the transformation effect. In addition, a very similar β-1, 6-glucan with side chains of β-Glc or α-Galρ which was purified from Lentinus edod es showed same activities with those from Amillariella Mellea. Our findings shed light on the action mode of β-1, 6-glucan in cancer immunotherapy.