Quantitative Formation of Monomeric G‐Quadruplex DNA from Multimeric Structures of c‐Myc Promoter Sequence. (11th May 2020)

• Record Type: Journal Article
• Authors: Rauser, Valerie; Weinhold, Elmar
• Is Part Of: Chembiochem. Volume 21:Number 17(2020)
• Page Start: 2445
• Page End: 2448
• Publication Date: 2020-05-11
• Subjects: DNA -- G-quadruplexes -- monomer -- Pu27 -- size-exclusion chromatography; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.202000159
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract: G‐Quadruplex (G4)‐forming DNA sequences have a tendency to form stable multimeric structures. This can be problematic for studies with synthetic oligodeoxynucleotides. Herein, we describe a method that quantitatively converts multimeric intermolecular structures of the Pu27 sequence from the c‐myc promoter into the desired monomeric G4 by alkaline treatment and refolding. Abstract : A note of caution : G‐Quadruplex (G4)‐forming DNA sequences have a tendency to form stable multimeric structures that are difficult to denature. This can be problematic for studies with synthetic oligodeoxynucleotides. We describe a method that quantitatively converts multimeric intermolecular structures of Pu27 sequence from c‐myc promoter into desired monomeric G4 by alkaline treatment and refolding.