Prevalence and characterization of Clostridium perfringens isolated from feces of captive cynomolgus monkeys (Macaca fascicularis). (August 2020)

• Record Type: Journal Article
• Authors: Koo, Bon-Sang; Hwang, Eun-Ha; Kim, Green; Park, Joon-Young; Oh, Hanseul; Lim, Kyung Seob; Kang, Philyong; Lee, Hwal-Yong; Jeong, Kang-Jin; Mo, Inpil; Villinger, Francois; Hong, Jung Joo
• Is Part Of: Anaerobe. Volume 64(2020)
• Publication Date: 2020-08
• Subjects: Clostridium perfringens -- Non-human primates -- Cynomolgus monkeys -- Acute gastric dilatation -- Antibiotic resistance -- Biofilm; Anaerobic infections -- Periodicals; Anaerobic bacteria -- Periodicals; Bacterial diseases -- Periodicals; Computer network resources; Anaerobic protozoa -- Periodicals; 579.3
• DOI: 10.1016/j.anaerobe.2020.102236
• Languages: English
• ISSNs: 1075-9964

Abstract

Abstract: Clostridium perfringens is ubiquitous in the environment and the gastrointestinal tract of warm-blooded animals. While part of the gut microbiome, abnormal growth of C. perfringens causes histotoxic, neurologic, and enteric diseases in a variety of animal species, including humans, due to the production of toxins. There is extremely limited information on C. perfringens infection in non-human primates. Presently, 10 strains were successfully isolated from 126 monkeys and confirmed by molecular and biochemical analyses. All isolates were genotype A based on molecular analysis. Alpha toxin was identified in all isolates. Beta 2 toxin was detected in only three isolates. No other toxins, including enterotoxin, beta, iota, epsilon, and net B toxin, were identified in any isolate. All isolates were highly susceptible to β-lactam antibiotics. Double hemolysis and lecithinase activity were commonly observed in all strains. Biofilm formation, which can increase antibiotic resistance, was identified in 90% of the isolates. The data are the first report the prevalence and characteristics of C. perfringens isolated from captive cynomolgus monkeys. Highlights: Ten Clostridium perfringens were successfully isolated from 126 cynomolgus monkeys. All isolates were classified into genotype A based on molecular analysis. Double hemolysis and lecithinase activity were commonly observed in all strains. Biofilm formation was identified in 90% of isolates. All isolates were highly susceptible to β-lactam antibiotics.