Natural photoswitches expose STIM1 activation steps. (September 2020)

• Record Type: Journal Article
• Authors: Derler, Isabella; Romanin, Christoph
• Is Part Of: Cell calcium. Volume 90(2020)
• Publication Date: 2020-09
• Subjects: CRAC channel -- Optogenetic engineering -- STIM1; Calcium -- Metabolism -- Periodicals; Vertebrates -- Physiology -- Periodicals; Calcium -- Physiological effect -- Periodicals; Cell physiology -- Periodicals; Calcium in the body -- Periodicals; 572.516
• DOI: 10.1016/j.ceca.2020.102240
• Languages: English
• ISSNs: 0143-4160

Abstract

Abstract: STIM1, an ER-located Ca2+ sensor, activates Orai1 channels upon Ca2+-storedepletion. Prior to this, STIM1 undergoes a sequence of conformational changes, which cannot be controlled individually with high spatiotemporal resolution. Ma et al. [1 ] used the power of optogenetic engineering to transfer light-sensitivity to STIM1 and precisely characterize individual STIM1 activation steps.