Contact co-culture of osteoblasts and sympathetic neuronal cells enhances osteoblast differentiation. Issue 4 (July 2020)

• Record Type: Journal Article
• Authors: Tomoda, Taketo; Sato, Tsuyoshi; Ikami, Eiji; Fukushima, Yosuke; Kokabu, Shoichiro; Yoda, Tetsuya
• Is Part Of: Journal of oral and maxillofacial surgery, medicine, and pathology. Volume 32:Issue 4(2020)
• Page Start: 307
• Page End: 312
• Publication Date: 2020-07
• Subjects: Sympathetic neuronal cell -- Osteoblast differentiation -- Contact co-culture -- Semaphorin 3A -- Bone morphogenic protein; Mouth -- Surgery -- Periodicals; Face -- Surgery -- Periodicals; Maxilla -- Surgery -- Periodicals; Oral medicine -- Periodicals; Mouth -- Diseases -- Pathogenesis -- Periodicals; Surgery, Oral -- Periodicals; Oral Medicine -- Periodicals; Pathology, Oral -- Periodicals; Face -- Surgery; Maxilla -- Surgery; Mouth -- Diseases -- Pathogenesis; Mouth -- Surgery; Oral medicine; Electronic journals -- Sciences; Electronic journals -- Medicine; Periodicals; 617.522059
• DOI: 10.1016/j.ajoms.2020.01.004
• Languages: English
• ISSNs: 2212-5566

Abstract

Abstract: Objective: Bone and nerve reconstruction is crucial for treating various diseases of the oral and maxillofacial region. However, the relationship between the bone and the nervous systems is not fully elucidated. Methods: Osteoblastic and sympathetic neuronal cell lines were maintained in contact co-culture. Quantitative reverse-transcription polymerase chain reaction (RT-qPCR), immunoblot analysis, RNA interference, alkaline phosphatase (ALP) activity, and mineralization assays were performed. Results: Contact co-culture enhanced osteoblast calcification, promoted ALP activity, and increased the expression of Bglap and Runx2. Knockdown of Sema3A by RNA interference did not completely inhibit RUNX2 expression in contact co-culture. Sympathetic neuronal cells produce BMP-2; Bmp2 expression was upregulated in contact co-culture. Conclusions: We showed that sympathetic neuronal cells promote osteoblast differentiation by contact co-culture with osteoblasts, partly via SEMA3A. BMP-2 from sympathetic neuronal cells may promote osteoblast differentiation in a contact co-culture system.