Biology, venom composition, and scorpionism induced by brazilian scorpion Tityus stigmurus (Thorell, 1876) (Scorpiones: Buthidae): A mini-review. (15th October 2020)

• Record Type: Journal Article
• Authors: Furtado, Allanny Alves; Daniele-Silva, Alessandra; Silva-Júnior, Arnóbio Antônio da; Fernandes-Pedrosa, Matheus de Freitas
• Is Part Of: Toxicon. Volume 185(2020)
• Page Start: 36
• Page End: 45
• Publication Date: 2020-10-15
• Subjects: Scorpion -- Tityus stigmurus, scorpion venom -- Scorpionism -- Neurotoxic peptide -- Antimicrobial peptide -- Buthidae; ACE angiotensin-converting enzyme -- AMP antimicrobial peptide -- ATCC American Type Culture Collection -- BK bradykinin -- bp base pairs -- B16–F10 murine melanoma cell line -- Ca+ monovalent calcium ion -- Ca2+ bivalent calcium ion -- CD circular dichroism -- CI50 cytotoxicity index -- cDNA complementary deoxyribonucleic acid -- Cl− monovalent chlorine ion -- Cu2+ bivalent copper ion -- DBPs disulfide-bridged peptides -- DM molecular dynamic -- h hours -- HEK human kidney cell line -- HeLa cervical adenocarcinoma -- HepG2 human liver cancer cell line -- HPLC/MS high-performance liquid chromatography coupled to mass spectrometry -- kDA kilodaltons -- kg kilogram -- K+ monovalent potassium ion -- KTx toxins that act on potassium channels -- LD50 lethal dose capable of killing 50% of the population -- Lys Lysina -- mg milligram -- Na+ monovalent sodium ion -- NDBPs non-disulfide-bridged peptides -- NIH/3T3 murine fibroblast cell line -- NO nitric oxide -- Panc 10.05 pancreas adenocarcinoma -- PAPE proline rich peptides -- pH hydrogenionic potential -- RAW macrophage murine cell line -- SiHa human cervical carcinoma cell line -- SINAN (Notification Disease Information System) -- T cells T lymphocytes -- TFE 2, 2, 2- trifluoroethanol -- Tyr Tyrosine -- UV ultraviolet -- 2D-LC/MS Two-Dimensional Liquid Chromatography Coupled with High-Resolution Mass Spectrometry Analysis -- 2D-PAGE two-dimensional polyacrylamide gel electrophoresis -- 786–0 human kidney cancer cell line -- α alpha -- β beta -- γ gama -- κ kappa -- μM micromolar -- °C degrees Celsius; Toxins -- Periodicals; Venom -- Periodicals; 615.9
• DOI: 10.1016/j.toxicon.2020.06.015
• Languages: English
• ISSNs: 0041-0101

Abstract

Abstract: Scorpionism is a serious public health problem in various regions of the world. In Brazil, a high number of accidents by scorpions have been reported. From 2014 to 2018, about 547, 000 cases were recorded, resulting in 466 deaths. The scorpion Tityus stigmurus is the predominant species in the northeast of Brazil, being responsible for most scorpionism cases in this region. With the aid of the transcriptomic approach of the venom gland of this species, components as neurotoxins, antimicrobials, metal chelating peptides and hypotensins, have been identified and characterized in silico, showing different biologic activity in vitro . In addition, the neuronal, pancreatic, renal, and enzymatic effects have been demonstrated for the crude T. stigmurus venom. Therefore, the T. stigmurus scorpion venom constitutes a rich arsenal of bioactive molecules with high potential for therapeutic and biotechnological application. Highlights: T. stigmurus is the major cause of accidents in northeastern Brazil. The venom is one of the most lethal for mices among Brazilian species. The venom causes neuronal, pancreatic, renal damage, and has enzymatic properties. The venom has neurotoxic, antimicrobial, hypotensive and metal chelating molecules. Components of the venom have evident biotechnological and therapeutic potential.