Effects of Queso Blanco cheese containing Bifidobacterium longum KACC 91563 on fecal microbiota, metabolite and serum cytokine in healthy beagle dogs. (August 2020)

• Record Type: Journal Article
• Authors: Park, Ho-Eun; Kim, Ye Jin; Kim, Minseok; Kim, HyeRan; Do, Kyung-Hyo; Kim, Jae Kwang; Ham, Jun-Sang; Lee, Wan-Kyu
• Is Part Of: Anaerobe. Volume 64(2020)
• Publication Date: 2020-08
• Subjects: Probiotics -- Queso blanco cheese -- Bifidobacterium longum -- KACC 91563 -- Microbiota -- Companion dogs; Anaerobic infections -- Periodicals; Anaerobic bacteria -- Periodicals; Bacterial diseases -- Periodicals; Computer network resources; Anaerobic protozoa -- Periodicals; 579.3
• DOI: 10.1016/j.anaerobe.2020.102234
• Languages: English
• ISSNs: 1075-9964

Abstract

Abstract: The study describes the effects of administration of Queso Blanco cheese containing Bifidobacterium longum on the fecal microbiota, metabolite and serum cytokine in healthy beagle dogs. Twelve healthy beagle dogs were randomly divided in three groups of four dogs each:a control group, not fed with any cheese, and groups fed with Queso Blanco cheese with B. longum KACC 91563 (QCB) or without B. longum (QC) for 8 weeks. Fecal microbiota was analyzed using a culture-based method and 16s rRNA gene sequencing. Serum cytokine levels, activation of natural killer cells, and proliferation of peripheral blood mononuclear cells were determined. SPME-GC-MS method was used to determine the concentrations of short chain fatty acids and indole in dog feces. Administration of QCB for 4 weeks significantly increased the Bifidobacterium . QCB supplementation for 8 weeks reduceds Enterobacteriaceae and Clostridium perfringens (p < 0.05). The abundance of Fusobacterium, Blautia and Collinesella in QCB group were reduced as compared with the control group. Serum TNF-α and IL-6 levels at 8 weeks significantly increased in QCB group as compared with QC group. There was no change in the concentrations of total short chain fatty acids by B. longum at 0 and 4 weeks. At week 8, the acetic acid, propionic acid and butyric acid of the QCB and QC groups were significantly decreased compared to the control group. In conclusion, our results demonstrate that administration of QCB had positive effects on fecal microbiota and immune response in beagle dogs. We suggest that Queso Blanco cheese containing B. longum KACC 91563 could be used as a functional food for companion animals. Graphical abstract: Image 1 Highlights: B. longum was used to evaluate effects of microbiota and immune response in beagle. B. longum decrease abundance of Enterobacteriaceae and C. perfringens in dogs. B. longum decrease Fusobacterium, Blautia and Collinesella on fecal microbiota. B. longum KACC 91563 increase the serum TNF-α and IL-6 of health beagle dogs.