Toward an evaluation of metabolite channeling in vivo. (August 2020)

• Record Type: Journal Article
• Authors: Obata, Toshihiro
• Is Part Of: Current opinion in biotechnology. Volume 64(2020)
• Page Start: 55
• Page End: 61
• Publication Date: 2020-08
• Subjects: Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.copbio.2019.09.013
• Languages: English
• ISSNs: 0958-1669

Abstract

Graphical abstract: Highlights: Intermediate metabolites are channeled between metabolic pathway enzymes. Metabolite channeling is a promising tool to regulate metabolic pathway fluxes. Metabolic flux analyses can capture channeling signatures in a metabolic network. Evaluation of channeling effects in vivo requires multiplex approaches. Abstract : Intermediates of metabolic pathways are sometimes contained within cavities of enzyme molecules and passed directly from active centers of one to next enzymes without diffusing into bulk matrix. This 'metabolite channeling' is postulated to have various advantages in enhancing and regulating pathway reactions and considered as a central unit to control metabolic network. Therefore, it has a strong potential in applications to metabolic engineering to enhance the production of desired molecules. Quantitative evaluation of the effects of metabolite channeling is crucial for its appropriate application and further understanding of its functions. In the present review, current approaches to demonstrate functional metabolite channeling will be reviewed and their extension toward quantitative evaluation of channeling effects in vivo will be discussed.