Potent‐By‐Design: Amino Acids Mimicking Porous Nanotherapeutics with Intrinsic Anticancer Targeting Properties. Issue 34 (19th July 2020)

• Record Type: Journal Article
• Authors: Wu, Zhuoran; Lim, Hong Kit; Tan, Shao Jie; Gautam, Archana; Hou, Han Wei; Ng, Kee Woei; Tan, Nguan Soon; Tay, Chor Yong
• Is Part Of: Small. Volume 16:Issue 34(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-07-19
• Subjects: cancer targeting -- large neutral amino acid transporter -- mesoporous silica nanoparticles -- nanotherapeutics -- reactive oxygen species; Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.202003757
• Languages: English
• ISSNs: 1613-6810

Abstract

Abstract: Exogenous sources of amino acids are essential nutrients to fuel cancer growth. Here, the increased demand for amino acid displayed by cancer cells is unconventionally exploited as a design principle to replete cancer cells with apoptosis inducing nanoscopic porous amino acid mimics (Nano‐PAAM). A small library consisting of nine essential amino acids nanoconjugates (30 nm) are synthesized, and the in vitro anticancer activity is evaluated. Among the Nano‐PAAMs, l ‐phenylalanine functionalized Nano‐PAAM (Nano‐pPAAM) has emerged as a novel nanotherapeutics with excellent intrinsic anticancer and cancer‐selective properties. The therapeutic efficacy of Nano‐pPAAM against a panel of human breast, gastric, and skin cancer cells could be ascribed to the specific targeting of the overexpressed human large neutral amino acid transporter SLC7A5 (LAT‐1) in cancer cells, and its intracellular reactive oxygen species (ROS) inducing properties of the nanoporous core. At the mechanistic level, it is revealed that Nano‐pPAAM could activate both the extrinsic and intrinsic apoptosis pathways to exert a potent "double‐whammy" anticancer effect. The potential clinical utility of Nano‐pPAAM is further investigated using an MDA‐MB‐231 xenograft in NOD scid gamma mice, where an overall suppression of tumor growth by 60% is achieved without the aid of any drugs or application of external stimuli. Abstract : Large‐neutral amino acid transporter‐1 targeting nanoscopic porous l‐phenylalanine functionalized amino acid mimics is a new class of nanotherapeutics with intrinsic anticancer and cancer‐selective properties. Its design principle is premised on cancer cells' auxotrophy to exogenous amino acids supply and the intracellular reactive oxygen species amplifying properties of the mesoporous silica core, leading to the self‐destruction of cancer cells.