A novel workflow for three-dimensional analysis of tumour cell migration. Issue 2 (14th February 2020)

• Record Type: Journal Article
• Authors: Ketchen, Sophie; Rohwedder, Arndt; Knipp, Sabine; Esteves, Filomena; Struve, Nina; Peckham, Michelle; Ladbury, John E.; Curd, Alistair; Short, Susan C.; Brüning-Richardson, Anke
• Is Part Of: Interface focus. Volume 10:Issue 2(2020)
• Publication Date: 2020-02-14
• Subjects: 3D -- tumour spheroid -- microscopy -- ImageJ -- cell migration; Physical sciences -- Periodicals; Life sciences -- Periodicals; 500
• DOI: 10.1098/rsfs.2019.0070
• Languages: English
• ISSNs: 2042-8898

Abstract

Abstract : The limitations of two-dimensional analysis in three-dimensional (3D) cellular imaging impair the accuracy of research findings in biological studies. Here, we report a novel 3D approach to acquisition, analysis and interpretation of tumour spheroid images. Our research interest in mesenchymal–amoeboid transition led to the development of a workflow incorporating the generation and analysis of 3D data with instant structured illumination microscopy and a new ImageJ plugin.