Multifunctional solvothermal carbon derived from alginate using 'water-in-deep eutectic solvents' for enhancing enzyme activity. Issue 67 (22nd July 2020)

• Record Type: Journal Article
• Authors: Yadav, Niketa; M., Manohara Halanur; Bisht, Meena; Nataraj, S. K.; Venkatesu, Pannuru; Mondal, Dibyendu
• Is Part Of: Chemical communications. Volume 56:Issue 67(2020)
• Page Start: 9659
• Page End: 9662
• Publication Date: 2020-07-22
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d0cc03866k
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : The use of a water-in-DES system for conversion of a seaweed biopolymer to a highly oxygenated functional carbon is reported for protein packaging with improved activity and stability. Abstract : Herein, the use of a "water-in-deep eutectic solvent (DES)" system has been shown as an alternative platform for the low temperature conversion of alginic acid (AA) to a multifunctional aliginate derived carbon (AAC) material with variable oxygen functionalities. Using the in-built oxygenated functionalities in AAC, cytochrome- c (Cyt- c ) was immobilised on the surface of AACs and the peroxidase activity of the enzyme was studied. Remarkably, the enzymatic activity was enhanced up to 5.5-fold compared to the native protein without compromising the structural stability of Cyt- c . Altogether, the present study demonstrates a sustainable process for the preparation of AACs and shows their beneficial effect on the activity and stability of Cyt- c, and thus, their application as a protein-friendly material for biotechnological applications.