Paper-based 1, 5-anhydroglucitol quantification using enzyme-based glucose elimination. Issue 17 (20th July 2020)

• Record Type: Journal Article
• Authors: Jang, Hyungjun; Oh, Jusung; Ki, Hangil; Kim, Min-Gon
• Is Part Of: Analyst. Volume 145:Issue 17(2020)
• Page Start: 5740
• Page End: 5743
• Publication Date: 2020-07-20
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/d0an00905a
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : This paper-based sensor quantified glucose and 1, 5-anhydroglucitol using enzyme-based colorimetric and elimination reaction in human urine. Abstract : The monosaccharide 1, 5-anhydroglucitol (1, 5-AG) is a known indicator of glucose levels. Conventional 1, 5-AG quantification methods with enzyme-based sensors using pyranose oxidase (PROD) require elimination of interference from the sample (a laborious and time-consuming process), as PROD cannot distinguish 1, 5-AG from other sugars. We developed a one-step paper-based sensor for detecting 1, 5-AG using glucose oxidase, catalase, and mutarotase that eliminates excess glucose, which interferes with 1, 5-AG detection. This sensor consists of two compartments for the quantification of glucose and 1, 5-AG and reflects the concentration of these targets after reaction with water or spiked human urine. The limit of detection of the sensor was 0.9 mg dL −1 for glucose and 3.2 μg mL −1 for 1, 5-AG.