A guideline for homology modeling of the proteins from newly discovered betacoronavirus, 2019 novel coronavirus (2019‐nCoV). Issue 9 (29th March 2020)

• Record Type: Journal Article
• Authors: Dong, Shengjie; Sun, Jiachen; Mao, Zhuo; Wang, Lu; Lu, Yi‐Lin; Li, Jiesen
• Other Names: Luo Guangxiang (George) guestEditor.; Ly Hinh guestEditor.; Gao Shou‐Jiang guestEditor.
• Is Part Of: Journal of medical virology. Volume 92:Issue 9(2020)
• Page Start: 1542
• Page End: 1548
• Publication Date: 2020-03-29
• Subjects: 2019‐nCoV -- BLAST algorithm -- CLUSTAL analysis -- coronavirus -- homology modeling -- MERS‐CoV -- SARS‐CoV -- sequence alignment; Virology -- Periodicals; 616
• DOI: 10.1002/jmv.25768
• Languages: English
• ISSNs: 0146-6615

Abstract

Abstract: During an outbreak of respiratory diseases including atypical pneumonia in Wuhan, a previously unknown β‐coronavirus was detected in patients. The newly discovered coronavirus is similar to some β‐coronaviruses found in bats but different from previously known SARS‐CoV and MERS‐CoV. High sequence identities and similarities between 2019‐nCoV and SARS‐CoV were found. In this study, we searched the homologous templates of all nonstructural and structural proteins of 2019‐nCoV. Among the nonstructural proteins, the leader protein (nsp1), the papain‐like protease (nsp3), the nsp4, the 3C‐like protease (nsp5), the nsp7, the nsp8, the nsp9, the nsp10, the RNA‐directed RNA polymerase (nsp12), the helicase (nsp13), the guanine‐N7 methyltransferase (nsp14), the uridylate‐specific endoribonuclease (nsp15), the 2'‐O‐methyltransferase (nsp16), and the ORF7a protein could be built on the basis of homology templates. Among the structural proteins, the spike protein (S‐protein), the envelope protein (E‐protein), and the nucleocapsid protein (N‐protein) can be constructed based on the crystal structures of the proteins from SARS‐CoV. It is known that PL‐Pro, 3CL‐Pro, and RdRp are important targets for design antiviral drugs against 2019‐nCoV. And S protein is a critical target candidate for inhibitor screening or vaccine design against 2019‐nCoV because coronavirus replication is initiated by the binding of S protein to cell surface receptors. It is believed that these proteins should be useful for further structure‐based virtual screening and related computer‐aided drug development and vaccine design. Highlights: High sequence identities between 2019‐nCoV and SARS‐CoV were found. Homology templates of all structural proteins of 2019‐nCoV were identified. Homology templates of all nonstructural proteins of 2019‐nCoV were identified.