High‐throughput in situ experimental phasing. Issue 8 (3rd August 2020)

• Record Type: Journal Article
• Authors: Lawrence, Joshua M.; Orlans, Julien; Evans, Gwyndaf; Orville, Allen M.; Foadi, James; Aller, Pierre
• Is Part Of: Acta crystallographica. Volume 76:Issue 8(2020)
• Page Start: 790
• Page End: 801
• Publication Date: 2020-08-03
• Subjects: in situ crystallography -- experimental phasing -- crystal soaking -- high throughput -- isomorphism; X-ray crystallography -- Periodicals; Crystallography -- Periodicals; Molecular biology -- Periodicals; Molecular structure -- Periodicals; Biomolecules -- Structure -- Periodicals; Cytology -- Periodicals; Biomolecules -- Structure; Crystallography; Cytology; Molecular biology; Molecular structure; X-ray crystallography; Periodicals; 548
• DOI: 10.1107/S2059798320009109
• Languages: English
• ISSNs: 2059-7983

Abstract

Abstract : A new procedure for experimental phasing at room temperature using diffraction data collected directly from the crystallization plates is described, demonstrated and tested. Abstract : In this article, a new approach to experimental phasing for macromolecular crystallography (MX) at synchrotrons is introduced and described for the first time. It makes use of automated robotics applied to a multi‐crystal framework in which human intervention is reduced to a minimum. Hundreds of samples are automatically soaked in heavy‐atom solutions, using a Labcyte Inc. Echo 550 Liquid Handler, in a highly controlled and optimized fashion in order to generate derivatized and isomorphous crystals. Partial data sets obtained on MX beamlines using an in situ setup for data collection are processed with the aim of producing good‐quality anomalous signal leading to successful experimental phasing.