LncRNA TTN‐AS1 acts as sponge for miR‐15b‐5p to regulate FBXW7 expression in ovarian cancer. (12th February 2020)

• Record Type: Journal Article
• Authors: Miao, Sheng; Wang, Jia; Xuan, Lili; Liu, Xiaojun
• Is Part Of: BioFactors. Volume 46:Number 4(2020)
• Page Start: 600
• Page End: 607
• Publication Date: 2020-02-12
• Subjects: FBXW7 -- miR‐15b‐5p -- ovarian cancer -- TTN‐AS1; Vitamins -- Physiological effect -- Periodicals; Trace elements -- Physiological effect -- Periodicals; Growth factors -- Physiological effect -- Periodicals; Plant growth promoting substances -- Physiological effect -- Periodicals; Biochemistry -- Periodicals; Nutritional Physiological Phenomena -- Periodicals; Trace Elements -- metabolism -- Periodicals; Vitamins -- metabolism -- Periodicals; Molecular Biology -- Periodicals; 612.399
• DOI: 10.1002/biof.1622
• Languages: English
• ISSNs: 0951-6433

Abstract

Abstract: Emerging evidence showed that long noncoding RNA (lncRNA) plays crucial roles in regulating various cancer biological behaviors. Titin‐antisense RNA1 (TTN‐AS1) has been reported to have crucial roles in cancers but its role in ovarian cancer remains unknown. The levels of TTN‐AS1, microNRA‐15b‐5p (miR‐15b‐5p), and F‐box and WD repeat domain containing 7 (FBXW7) in ovarian cancer cells were measured by quantitative reverse‐transcription PCR. Targets for TTN‐AS1 and miR‐15b‐5p were predicted by bioinformatic tools, and validated by luciferase activity reporter assay. Cell proliferation, colony formation, and cell apoptosis were analyzed with cell counting kit‐8 assay, colony formation assay, and flow cytometry. Correlation of TTN‐AS1 and FBXW7 was analyzed at gene expression profiling interactive analysis. TTN‐AS1 was found decreased expression in ovarian cancer tissues and cells. Dual‐luciferase activity validated TTN‐AS1 and FBXW7 shared binding site in miR‐15b‐5p. Functional assays showed TTN‐AS1 overexpression inhibits ovarian cancer cell proliferation, colony formation but promotes apoptosis. Rescue experiments showed that knockdown of FBXW7 could partially counteracted the effects of TTN‐AS1 overexpression on ovarian cancer cell behaviors. Our results indicated that the TTN‐AS1/miR‐15b‐5p/FBXW7 axis identified in this work could help to identify treatment biomarkers for ovarian cancer.