Microbial production of 4‐amino‐1‐butanol, a four‐carbon amino alcohol. Issue 9 (3rd June 2020)

• Record Type: Journal Article
• Authors: Prabowo, Cindy Pricilia Surya; Shin, Jae Ho; Cho, Jae Sung; Chae, Tong Un; Lee, Sang Yup
• Is Part Of: Biotechnology and bioengineering. Volume 117:Issue 9(2020)
• Page Start: 2771
• Page End: 2780
• Publication Date: 2020-06-03
• Subjects: 4‐amino‐1‐butanol -- amino alcohol -- Corynebacterium glutamicum -- metabolic engineering -- putrescine; Biotechnology -- Periodicals; Bioengineering -- Periodicals; 660.6
• DOI: 10.1002/bit.27438
• Languages: English
• ISSNs: 0006-3592

Abstract

Abstract: 4‐Amino‐1‐butanol (4AB) serves as an important intermediate compound for drugs and a precursor of biodegradable polymers used for gene delivery. Here, we report for the first time the fermentative production of 4AB from glucose by metabolically engineered Corynebacterium glutamicum harboring a newly designed pathway comprising a putrescine (PUT) aminotransferase (encoded by ygjG ) and an aldehyde dehydrogenase (encoded by yqhD ) from Escherichia coli, which convert PUT to 4AB. Application of several metabolic engineering strategies such as fine‐tuning the expression levels of ygjG and yqhD, eliminating competing pathways, and optimizing culture condition further improved 4AB production. Fed‐batch culture of the final metabolically engineered C. glutamicum strain produced 24.7 g/L of 4AB. The strategies reported here should be useful for the microbial production of primary amino alcohols from renewable resources. Abstract : The authors report the production of 4‐amino‐1‐butanol (4AB) by metabolically engineered Corynebacterium glutamicum . 4AB serves as an important raw compound for various applications in the pharmaceutical field. To produce 4AB, a synthetic pathway comprising Escherichia coli putrescine (PUT) aminotransferase ( ygjG ) and aldehyde dehydrogenase ( yqhD ) was introduced to a PUT producing C. glutamicum strain. The titer of 4AB was improved by fine‐tuning the expression levels of ygjG and yqhD, deleting competing pathways, and optimizing culture conditions.