A potential alternative to traditional antibiotics in aquaculture: Yeast glycoprotein exhibits antimicrobial effect in vivo and in vitro on Aeromonas caviae isolated from Carassius auratus gibelio. Issue 3 (24th March 2020)

• Record Type: Journal Article
• Authors: Wu, Ronghua; Shen, Junyu; Tian, Dandan; Yu, Jiaqian; He, Tao; Yi, Jianhua; Li, Yun
• Is Part Of: Veterinary medicine and science. Volume 6:Issue 3(2020)
• Page Start: 639
• Page End: 648
• Publication Date: 2020-03-24
• Subjects: Aeromonas caviae -- antimicrobial drugs -- Carassius auratus gibelio -- yeast glycoprotein; Veterinary medicine -- Periodicals; Animal Diseases; Veterinary medicine; Periodicals; Periodicals; 636.08905
• DOI: 10.1002/vms3.253
• Languages: English
• ISSNs: 2053-1095

Abstract

Abstract: In aquaculture, antibiotics are commonly used to provide protection against pathogens; however, this practice has become controversial due to increased occurrences of microbial resistance, and alternatives are needed. This study aimed to investigate the antimicrobial activity of yeast glycoprotein (YG) against Aeromonas caviae . Pathogens were isolated from liver of diseased Carassius auratus gibelio . Based on morphological and biochemical analysis, together with 16S rRNA gene sequencing, the isolated strains were identified as A. caviae and concluded as clones of a single strain and named L2. Further pathogenicity analysis revealed that A. caviae possessed β‐haemolysis, and its median lethal dose for C. gibelio was 1.33 × 10 6 CFU/ml. Hepatic adenylate kinase and pyruvate kinase activities of C. gibelio were inhibited post– A. caviae infection. Antimicrobial drug test suggested that A. caviae was a multidrug‐resistant organism but could be inhibited by YG in vitro. Minimum inhibitory and bactericidal concentration of YG was 83.3 mg/ml and 166.7 mg/ml, respectively. Microbiota sequencing results showed that YG supplement could obviously decrease the relative abundance of Aeromonas and increase the microbial diversity. Our study revealed that A. caviae from C. gibelio was a multidrug‐resistant bacteria strain, and could be significantly inhibited by YG in vivo and in vitro, thus providing important insights into ecological control and pathogenesis of A. caviae in aquaculture. Abstract : This is the first report that C. gibelio is susceptible to Aeromonas caviae infection. yeast glycoprotein (YG) supplement could increase microbial diversity and decrease abundance of Aeromonas. Aeromonas caviae was multi‐antibiotic and could be inhibited by YG in vivo.