Dicer and Drosha expression in patients with nephrotic syndrome. (15th May 2020)

• Record Type: Journal Article
• Authors: Hejazian, Seyyedeh Mina; Rahbar Saadat, Yalda; Bahmanpour, Zahra; Hosseiniyan Khatibi, Seyed Mahdi; Ardalan, Mohammadreza; Zununi Vahed, Sepideh
• Is Part Of: BioFactors. Volume 46:Number 4(2020)
• Page Start: 645
• Page End: 652
• Publication Date: 2020-05-15
• Subjects: Dicer -- Drosha -- microRNA biogenesis -- nephrotic syndrome -- podocytopathy; Vitamins -- Physiological effect -- Periodicals; Trace elements -- Physiological effect -- Periodicals; Growth factors -- Physiological effect -- Periodicals; Plant growth promoting substances -- Physiological effect -- Periodicals; Biochemistry -- Periodicals; Nutritional Physiological Phenomena -- Periodicals; Trace Elements -- metabolism -- Periodicals; Vitamins -- metabolism -- Periodicals; Molecular Biology -- Periodicals; 612.399
• DOI: 10.1002/biof.1638
• Languages: English
• ISSNs: 0951-6433

Abstract

Abstract: Podocytes play an essential role in the regulation of glomerular filtration and the appropriate function of the kidney. Podocytes injury is involved in the pathogenesis of nephrotic syndrome (NS), a common renal glomerulus dysfunction characterized by proteinuria. Some in vivo studies in Dicer/Drosha knockout mice indicate the importance of Dicer, Drosha, and microRNAs (miRNAs) in the pathogenesis of NS. In the present study, the expression levels of Dicer and Drosha along with miR‐30 family, miR‐186, miR‐193, and miR‐217 were evaluated in peripheral blood mononuclear cell samples of patients with NS ( N = 60) using real‐time PCR. Dicer expression level in NS patients was significantly upregulated when compared to healthy controls ( p = .008). No significant change was observed in the Drosha expression level in the NS group. Upregulated levels of the studied microRNAs were observed in NS group in comparison to controls, the miR‐30c‐5p ( p = .005) and miR‐193‐3p ( p = .041) were statistically significant. In conclusion, dysregulation in expression level of Dicer and Drosha and consequently, alteration in miRNA levels are involved in the pathophysiology of NS.