Affinity purification of multifunctional oligomeric ligands synthesized via controlled radical polymerization. Issue 26 (10th June 2020)

• Record Type: Journal Article
• Authors: Hoshino, Yu; Shimohara, Shinnosuke; Wada, Yusuke; Nakamoto, Masahiko; Miura, Yoshiko
• Is Part Of: Journal of materials chemistry. Volume 8:Issue 26(2020)
• Page Start: 5597
• Page End: 5601
• Publication Date: 2020-06-10
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/d0tb00849d
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : Abiotic oligomeric ligands with a strong affinity for a target peptide sequence were isolated by affinity purification from a pool of 30-mer acrylic random ter-oligomers that were synthesized via a controlled radical polymerization process. Abstract : Abiotic oligomeric ligands with a strong affinity for a target peptide sequence were isolated by affinity purification from a pool of 30-mer acrylic random ter-oligomers that were synthesized via a controlled radical polymerization process. Our results indicate that the oligomeric ligands with suitable sequence and/or stereochemical configurations for the target can be isolated from as-polymerized random co-polymers based on the affinity to the target. This process will be a powerful tool for the development of stable and inexpensive ligands that can be used to detect, neutralize and purify proteins with a target epitope sequence.