A biomimetic yeast shell vaccine coated with layered double hydroxides induces a robust humoral and cellular immune response against tumors. Issue 8 (8th July 2020)

• Record Type: Journal Article
• Authors: Liu, Dong-qun; Lu, Shuai; Zhang, Lun; Zhang, Ling-xiao; Ji, Mei; Liu, Xiao-Ge; Yu, Zhuo; Liu, Rui-tian
• Is Part Of: Nanoscale advances. Volume 2:Issue 8(2020)
• Page Start: 3494
• Page End: 3506
• Publication Date: 2020-07-08
• Subjects: 620.5
• DOI: 10.1039/d0na00249f
• Languages: English
• ISSNs: 2516-0230

Abstract

Abstract : Schematic illustration of the LDH nanoparticle-coated yeast shell vaccine and immune activation. Abstract : Enhancing both the humoral and cellular immune response for tumor vaccination remains a challenge. Inspired by natural pathogen structures, we took β-glucan particles derived from a baker's yeast cell shell (YS) as a vaccine carrier and danger signal for dendritic cells (DCs), and coated the YS with catanionic layered double hydroxides (LDH) by electrostatic adsorption to form a biomimetic yeast cell particle (YSL). Our experimental results showed that the YSL vaccine efficiently targeted antigen-presenting cells (APCs) and remarkably enhanced antigen cross-presentation, and strongly improved the activation and maturation of DCs. Moreover, the YSL vaccine elicited an extremely high antibody titer and strong antigen-specific cytotoxic T lymphocyte together with mixed Th1/Th17 cellular immune responses and induced marked prophylactic and therapeutic effects against E.G7-OVA tumors in mouse models. These results suggest that YSL, integrating a yeast shell to mimic natural pathogens and LDH with high antigen-loading capacity and lysosome escape, is a promising tumor vaccine platform for rapid, effective and strong induction of both humoral and cellular immune responses.