Cell-mimic polymersome-shielded islets for long-term immune protection of neonatal porcine islet-like cell clusters. Issue 12 (28th February 2020)

• Record Type: Journal Article
• Authors: Kim, Hyun-Ouk; Lee, Sang Hoon; Na, Woonsung; Lim, Jong-Woo; Park, Geunseon; Park, Chaewon; Lee, Hwunjae; Kang, Aram; Haam, Seungjoo; Choi, Inho; Kang, Jung-Taek; Song, Daesub
• Is Part Of: Journal of materials chemistry. Volume 8:Issue 12(2020)
• Page Start: 2476
• Page End: 2482
• Publication Date: 2020-02-28
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c9tb02270h
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : A PSome-shielded NPCC is achieved by binding the surface amine group of NPCCs and various functional groups of the PSome. The coating utilizes interaction of the NPCC surface and PSomes that have covalent bonds, electrostatic interactions, and hydrogen bonds. Also, PSomes coated NPCCs have selective permeability necessary for NPCC survival, function and immune protection. Abstract : Although islet cell transplantation has emerged as a promising treatment for type 1 diabetes, it remains an unmet clinical application due to the need for immunosuppression to prevent islet elimination and autoimmunity. To solve these problems, we developed novel nanoencapsulation of neonatal porcine islet-like cell clusters (NPCCs) with cell-mimic polymersomes (PSomes) based on PEG- b -PLA (poly(ethylene glycol)- b -poly(dl -lactic acid)). To accomplish this, we first formulated NHS-, NH2 -, COOH-, and m(methoxy)-PSomes. This coating utilizes interactions involving NPCC surfaces and PSomes that have covalent bonds, electrostatic interactions, and hydrogen bonds. We extended the range of applicability by comparing the binding affinity of electrostatic attraction and hydrogen bonding, as well as covalent bonds. Our protocol can be used as an efficient hydrogen bonding method because it reduces cell membrane damage as well as the use of covalent bonding methods. We verified the selective permeability of NHS-, NH2 -, COOH-, and m-PSome-shielded NPCCs. Furthermore, we showed that a novel nanoencapsulation did not affect insulin secretion from NPCCs. This study offers engineering advances in islet encapsulation technologies to be used for cell-based transplantation therapies.