Rapid and accurate detection of Escherichia coli O157:H7 in beef using microfluidic wax-printed paper-based ELISA. Issue 8 (11th March 2020)

• Record Type: Journal Article
• Authors: Zhao, Yanan; Zeng, Dexin; Yan, Chao; Chen, Wei; Ren, Jianluan; Jiang, Yuan; Jiang, Luyan; Xue, Feng; Ji, Dejun; Tang, Fang; Zhou, Mingqiang; Dai, Jianjun
• Is Part Of: Analyst. Volume 145:Issue 8(2020)
• Page Start: 3106
• Page End: 3115
• Publication Date: 2020-03-11
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/d0an00224k
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : Escherichia coli O157:H7 is a severe foodborne pathogen. Paper-based ELISA can rapidly and accurately detect E.coli O157:H7 in beef. The method has good sensitivity, specificity and repeatability. It is suitable for point-of-care testing and offers new ideas for the detection of other foodborne pathogens. Abstract : Escherichia coli O157:H7 is a severe foodborne pathogen that causes lots of life-threatening diseases. In the search for a rapid, sensitive, portable and low-cost method to detect this pathogen, we developed a wax-printed paper-based enzyme-linked immunosorbent assay (P-ELISA) based on microfluidic paper-based analytical devices (μPADs), with the whole operation time being less than 3 h and only needing 5 μl samples for detection. The limit of detection (LOD) of E. coli O157:H7 reached 10 4 CFU ml −1, which is an order of magnitude higher than that of conventional ELISA (C-ELISA). The LOD in artificially contaminated beef samples is 1 CFU per 25 g after enriching the culture for 8 h. This method is superior to the molecular biology method in detection sensitivity and superior to C-ELISA and the national standard method in detection time and cost. Thus, the established P-ELISA method has good sensitivity, specificity and repeatability. It can be suitable for point-of-care testing without expensive and bulky instruments and can also provide a platform for detecting other pathogens, especially in areas that lack advanced clinical equipment.