Improving resolving ability of expansion microscopy by varying crosslinker concentration. Issue 30 (13th March 2020)

• Record Type: Journal Article
• Authors: Hu, Yanlei; Chu, Ximing; Chen, Ting-ting; Pan, Qingshan; Liu, Chang; Yi, Jintao; Chu, Xia
• Is Part Of: Chemical communications. Volume 56:Issue 30(2020)
• Page Start: 4176
• Page End: 4179
• Publication Date: 2020-03-13
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d0cc00052c
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Here, we systematically investigated the performance of expansion microscopy (ExM) with different crosslinker concentrations. Abstract : Here, we systematically investigated the performance of expansion microscopy (ExM) with different crosslinker concentrations. We modified ExM with 0.06% N, N ′-methylenebisacrylamide (MBAA) (termed 0.06%-MBAA ExM), increased the expansion factor to 5.7 and achieved a lateral resolution of ∼50 nm with a common confocal microscope.