New simple molecular fluorescent probes for rapid and highly selective sensing of hydrazine by aggregate-induced emission. Issue 13 (26th May 2020)

• Record Type: Journal Article
• Authors: Pavankumar, B. B.; Ranjan, Prabodh; Jha, Prakash C.; Sivaramakrishna, Akella
• Is Part Of: Analyst. Volume 145:Issue 13(2020)
• Page Start: 4615
• Page End: 4626
• Publication Date: 2020-05-26
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/d0an00601g
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : A rapid, selective, and sensitive method is developed and employed in the detection of hydrazine in water and living cells. Abstract : With an aim towards the design of efficient and straightforward fluorescent probes for hydrazine, the synthesis of (2-acetoxyaryl) methylene diacetate derivatives (1–4 ) was carried out by reacting substituted aromatic α-hydroxy aldehydes with acetyl chloride and sodium acetate in excellent yields. As a preliminary investigation, the ability of probe 1 was examined for the detection of substituted aliphatic and aromatic amines, amino acids, and other ions in Britton–Robinson buffer solution (50 mM, water/ethanol v/v of 99/1 at pH 7.4). Probe 1 selectively exhibited an intense blue fluorescence with hydrazine in less than 2 minutes, whereas light green or no fluorescence was noticed with substituted amines and amino acids. Among all the probes employed (1–4 ) in the present study, probes 1 and 2 were found efficient towards the rapid detection of hydrazine. Furthermore, the fluorescence sensing ability of probes 1 and 2 was tested not only under varying pH conditions but also by varying water-fraction from 0–99%. Moreover, the detection limits of hydrazine using 1 and 2 were found as 8.4 and 8.7 ppb, respectively, which is less than the acceptable limit as per the standards of the US Environment Protection Agency. In this contribution, the probes 1 and 2 demonstrate rapid, selective, sensitive, and ratiometric detection of highly toxic hydrazine by OFF–ON fluorescence switch in water samples as well as living cells.