Enzymatic synthesis of 2-hydroxy-4H-quinolizin-4-one scaffolds by integrating coenzyme a ligases and a type III PKS from Huperzia serrata. Issue 40 (22nd June 2020)

• Record Type: Journal Article
• Authors: Wang, Juan; Ding, Ning; Wu, Yun; Shi, Xiaoping; Qi, Bowen; Liu, Xiao; Wang, Xiaohui; Li, Jun; Tu, Pengfei; Shi, Shepo
• Is Part Of: RSC advances. Volume 10:Issue 40(2020)
• Page Start: 23566
• Page End: 23572
• Publication Date: 2020-06-22
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d0ra04133e
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : One-pot enzymatic synthesis of 2-hydroxy-4 H -quinolizin-4-one scaffolds was developed by integrating three enzymes PcPCL, AtMatB, and HsPKS3. Abstract : 2-Hydroxy-4 H -quinolizin-4-one scaffolds were enzymatically synthesized by integrating three enzymes including phenylacetate-CoA ligase (PcPCL) from an endophytic fungus Penicillium chrysogenum MT-12, malonyl-CoA synthase (AtMatB) from Arabidopsis thaliana, and a type III polyketide synthase (HsPKS3) from Chinese club moss Huperzia serrata . The findings paved the way to produce these kinds of structurally interesting alkaloids by engineered microorganisms.