A sustainable strategy for the assembly of Glypromate® and its structurally-related analogues by tandem sequential peptide coupling. Issue 11 (15th May 2020)

• Record Type: Journal Article
• Authors: Sampaio-Dias, Ivo E.; Pinto da Silva, Luís; Silva, Sandra G.; García-Mera, Xerardo; Rodríguez-Borges, José E.
• Is Part Of: Green chemistry. Volume 22:Issue 11(2020)
• Page Start: 3584
• Page End: 3596
• Publication Date: 2020-05-15
• Subjects: Environmental chemistry -- Industrial applications -- Periodicals; Environmental management -- Periodicals; 660
• DOI: 10.1039/d0gc00143k
• Languages: English
• ISSNs: 1463-9262

Abstract

Abstract : A greener and efficient one-pot methodology for the assembly of Glypromate® and its structurally-related analogues by tandem sequential peptide coupling. Abstract : This work describes an improved and greener methodology of solution-phase synthesis for the preparation of Glypromate® (glycyl-l -prolyl-l -glutamic acid, GPE), a potent neuropeptide for applications in neurodegenerative conditions such as Huntington's, Parkinson's and Alzheimer's diseases. This protocol comprises the assembly of the perbenzylated form of Glypromate® [Cbz-Gly-Pro-Glu(OBn)-OBn (5 )] from l -proline. Following a tandem sequential peptide coupling strategy, two chemoselective peptide bonds are formed without the need for purifying the intermediates ensuing a one-pot fashion synthesis. EcoScale score and E-factor were selected as the green metrics to assess the environmental impact of the preparation of tripeptide 5 using this protocol. After optimization and application of greener conditions, intermediate 5 was obtained with 95% global yield and 99% purity (NMR, HRMS, and rp-HPLC), with excellent final EcoScale score of 75 out of 100 and global E-factor of 1.8. Glypromate® is achieved by removing N- and C-protecting groups by hydrogenolysis using Pd/C as the catalyst in 98% yield, avoiding chromatographic techniques. Moreover, the protocol ensures stereochemical integrity (determined by VT-NMR and rp-HPLC) and was also successfully applied for the preparation of structurally-related Glypromate® analogues with higher degree of molecular complexity compatible with functionalized amino acids with different side chains. For the first time a one-pot protocol for the assembly of tripeptides with the removal of protecting groups in the same reaction vessel is reported.