Injectable hydrogels based on MPEG–PCL–RGD and BMSCs for bone tissue engineering. (1st July 2020)

• Record Type: Journal Article
• Authors: Kim, Hyun Joo; You, Su Jung; Yang, Dae Hyeok; Eun, Jin; Park, Hae Kwan; Kim, Moon Suk; Chun, Heung Jae
• Is Part Of: Biomaterials science. Volume 8:Number 15(2020)
• Page Start: 4334
• Page End: 4345
• Publication Date: 2020-07-01
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1039/d0bm00588f
• Languages: English
• ISSNs: 2047-4830

Abstract

Abstract : The aim of this study was to investigate the osteogenic potential of BMSCs seeded on RGD-conjugated methoxy polyethylene glycol-polycaprolactone (MP–RGD) in vitro and in vivo . Abstract : The aim of this study was to investigate the osteogenic potential of bone marrow-derived mesenchymal stem cells (BMSCs) seeded on novel thermosensitive in situ forming hydrogel systems comprising methoxy polyethylene glycol-polycaprolactone (MP) and RGD-conjugated MP (MP–RGD) in vitro and in vivo . Real-time polymerase chain reaction (PCR) together with immunofluorescence staining revealed the strong expression of osteogenic markers (collagen 1 and osteocalcin) of BMSCs in MP/MP–RGD samples compared to MP samples. PCR array testing also showed the upregulation of the interconnected signaling networks regulating cell proliferation and differentiation, which was further verified through the Kyoto Encyclopedia of Genes and Genomes pathway analysis. Histological findings and computed tomographic analysis demonstrated that the MP/MP–RGD hydrogel dramatically promoted new bone formation in a rabbit calvarial defect model. In conclusion, this hydrogel appears to elicit cellular behaviors desired for bone tissue regeneration.