Butein, isoliquiritigenin, and scopoletin attenuate neurodegeneration via antioxidant enzymes and SIRT1/ADAM10 signaling pathway. Issue 28 (27th April 2020)

• Record Type: Journal Article
• Authors: Gay, Naw Hser; Suwanjang, Wilasinee; Ruankham, Waralee; Songtawee, Napat; Wongchitrat, Prapimpun; Prachayasittikul, Virapong; Prachayasittikul, Supaluk; Phopin, Kamonrat
• Is Part Of: RSC advances. Volume 10:Issue 28(2020)
• Page Start: 16593
• Page End: 16606
• Publication Date: 2020-04-27
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c9ra06056a
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Neuronal cells exposed to H2 O2 may undergo increase ROS, reduction in cell viability and cell death. Butein, isoliquiritigenin, and scopoletin ameliorated H2 O2 -induced neurotoxicity by reducing ROS, balancing antioxidants and activating SIRT1-FoxO3a-ADAM10 pathway. Abstract : Neuronal cell death is a key feature of neurodegenerative disorders such as Parkinson's and Alzheimer's diseases. Plant polyphenols, namely butein, isoliquiritigenin, and scopoletin, have been shown to exhibit various biological activities including anti-inflammatory, antimicrobial, and antioxidant activities. Herein, butein, isoliquiritigenin, and scopoletin were explored for their neuroprotective properties against oxidative stress-induced human dopaminergic SH-SY5Y cell death. The cells exposed to hydrogen peroxide (H2 O2 ) revealed a reduction in cell viability and increases in apoptosis and levels of reactive oxygen species (ROS). Interestingly, pretreatment of SH-SY5Y cells with 5 μM of butein, isoliquiritigenin, or scopoletin protected against the cell death induced by H2 O2, and decreased the levels of apoptotic cells and ROS. In addition, the levels of SIRT1, FoxO3a, ADAM10, BCL-2, and antioxidant enzymes (catalase and SOD2) were maintained in the cells pretreated with butein, isoliquiritigenin, or scopoletin before H2 O2 treatment compared to cells without pretreatment and the reference (resveratrol). Molecular docking analysis revealed that the interactions between the activator-binding sites of SIRT1 and the phenolic compounds were similar to those of resveratrol. Taken together, the data suggest that these polyphenolic compounds could be potential candidates for prevention and/or treatment of neurodegeneration.