Amino-modified kraft lignin microspheres as a support for enzyme immobilization. Issue 36 (4th June 2020)

• Record Type: Journal Article
• Authors: Bebić, Jelena; Banjanac, Katarina; Rusmirović, Jelena; Ćorović, Marija; Milivojević, Ana; Simović, Milica; Marinković, Aleksandar; Bezbradica, Dejan
• Is Part Of: RSC advances. Volume 10:Issue 36(2020)
• Page Start: 21495
• Page End: 21508
• Publication Date: 2020-06-04
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d0ra03439h
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : The active biocatalyst systems were developed by immobilizing β-galactosidase from A. oryzae and laccase from M. thermophila expressed in A. oryzae (Novozym®51003) onto amino-modified microspheres based on bio-waste derived material, such as kraft lignin. Abstract : In this research, it has been demonstrated that amino-modified microspheres (A-LMS) based on bio-waste derived material, such as kraft lignin, have good prospects in usage as a support for enzyme immobilization, since active biocatalyst systems were prepared by immobilizing β-galactosidase from A. oryzae and laccase from M. thermophila expressed in A. oryzae (Novozym® 51003) onto A-LMS. Two types of A-LMS were investigated, with different emulsifier concentrations (5 wt% and 10 wt%), and microspheres produced using 5 wt% of emulsifier (A-LMS_5) showed adequate pore shape, size and distribution for enzyme attachment. The type of interactions formed between enzymes (β-galactosidase and laccase) and A-LMS_5 microspheres demonstrated that β-galactosidase is predominantly attached via electrostatic interactions while attachment of laccase is equally governed by electrostatic and hydrophobic interactions. Furthermore, the A-LMS_5-β-galactosidase exhibited specificity towards recognized prebiotics (galacto-oligosaccharides (GOS)) synthesis with 1.5-times higher GOS production than glucose production, while for environmental pollutant lindane degradation, the immobilized laccase preparation exhibited high activity with a minimum remaining lindane concentration of 22.4% after 6 days. Thus, this novel enzyme immobilization support A-LMS_5 has potential for use in green biotechnologies.