Studies of the labile lead pool using a rhodamine-based fluorescent probe. Issue 5 (28th April 2020)

• Record Type: Journal Article
• Authors: Zhu, Jianping; Yeo, Jia Hao; Bowyer, Amy A.; Proschogo, Nicholas; New, Elizabeth J.
• Is Part Of: Metallomics. Volume 12:Issue 5(2020)
• Page Start: 644
• Page End: 648
• Publication Date: 2020-04-28
• Subjects: Metals -- Physiological effect -- Periodicals; 572.51
• DOI: 10.1039/d0mt00056f
• Languages: English
• ISSNs: 1756-5901

Abstract

Abstract : A rhodamine-based fluorescent lead probe reports on the labile lead pool within cells. Abstract : Lead is a heavy metal which has long been known to have toxic effects on the body. However, much remains to be learnt about the labile lead pool and cellular uptake of lead. We report here RPb1 that undergoes a 100-fold increase in fluorescence emission in the presence of Pb 2+, and which can be applied to study the labile lead pool within cells. We demonstrate the capacity of RPb1 for investigating labile lead pool in DLD-1 cells and changes in labile lead during differentiation of K562 cells.