Fast and precise quantification of serum biomarkers and simultaneous recognition of multiple diseases enabled by a stable isotope-labelled peptides assisted high-throughput MRM strategy. Issue 15 (17th July 2020)

• Record Type: Journal Article
• Authors: Hu, Anqi; Wang, Zhenxin; Xie, Juanjuan; Lin, Ling; Yang, Pengyuan; Zhang, Lei; Shen, Huali; Cao, Yi-ou
• Is Part Of: Analyst. Volume 145:Issue 15(2020)
• Page Start: 5299
• Page End: 5306
• Publication Date: 2020-07-17
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/d0an01100b
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : We constructed an MS strategy that enables fast and precise quantification of serum biomarkers through coupling high-throughput scheduled MRM strategy with stable isotope labelled peptides panel and simultaneous recognition of multiple diseases. Abstract : Serum/plasma holds promise as an important source of disease-related proteins and even biomarkers in clinical practice. However, the discovery of biomarker candidates in serum/plasma remains challenging. In this study, we constructed an MS strategy that enables the fast and precise quantification of serum biomarkers through coupling a high-throughput scheduled MRM strategy with a stable isotope-labelled (SIL) peptide panel from more than 500 plasma proteins as internal standards. With this strategy, we discovered relevant serum proteins of atherosclerosis (AS), lung cancer (LC) and breast cancer (BC), which can simultaneously recognize these diseases. The results indicate that the powerful strategy we constructed has the potential for serum biomarker screening and disease detection.