Photo-reactive oligodeoxynucleotide-embedded nanovesicles (PROsomes) with switchable stability for efficient cellular uptake and gene knockdown. Issue 66 (17th July 2020)

• Record Type: Journal Article
• Authors: Kim, Beob Soo; Naito, Mitsuru; Kamegawa, Rimpei; Kim, Hyun Jin; Iizuka, Ryo; Funatsu, Takashi; Ueno, Shingo; Ichiki, Takanori; Kishimura, Akihiro; Miyata, Kanjiro
• Is Part Of: Chemical communications. Volume 56:Issue 66(2020)
• Page Start: 9477
• Page End: 9480
• Publication Date: 2020-07-17
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d0cc01750g
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A photo-responsive nanovesicle (PROsome) is fabricated via polyion complex between PEG-polypeptides and photo-reactive/antisense oligodeoxynucleotides (PROs/ASOs), eliciting the switchable stability for efficient cellular uptake and gene knockdown. Abstract : A photo-responsive nanovesicle is fabricated by polyion complex (PIC) formation between poly(ethylene glycol) (PEG)- block -polypeptides and photo-reactive oligodeoxynucleotides (PROs)/anti-sense oligonucleotides (ASOs). The ultraviolet (UV) light triggers reversible crosslinking between PROs and ASOs in the vesicular membrane, providing the nanovesicle with switchable stability under physiological conditions. The resulting nanovesicle allows efficient cellular internalization, leading to significant UV-triggered gene knockdown in cultured cells.