A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems. Issue 40 (23rd June 2020)

• Record Type: Journal Article
• Authors: Popescu, Codruţa C.; Stoian, Marius C.; Cucos, Lia-Maria; Coman, Anca G.; Radoi, Antonio; Paun, Anca; Hădade, Niculina D.; Gautier, Arnaud; Popescu, Costin-Ioan; Matache, Mihaela
• Is Part Of: RSC advances. Volume 10:Issue 40(2020)
• Page Start: 23931
• Page End: 23935
• Publication Date: 2020-06-23
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d0ra02382e
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : We describe the synthesis of a novel polyamino polycarboxylic ligand, its ability to coordinate metal-ions and attachment to a solid support designed for protein purification through Immobilised Metal-ion Affinity Chromatography (IMAC). Abstract : We describe the synthesis of a novel polyamino polycarboxylic ligand, its ability to coordinate metal-ions and attachment to a solid support designed for protein purification through Immobilised Metal-ion Affinity Chromatography (IMAC). The resin was found to be highly efficient for purification of His-tagged HCV E2 glycoproteins expressed in 293T mammalian cells.