Electrochemical detection of bovine immunoglobulins G to determine passive transfer of antibodies to calves. Issue 21 (27th May 2020)

• Record Type: Journal Article
• Authors: Robinson, Caoimhe; Creedon, Niamh; Sayers, Riona; Kennedy, Emer; O'Riordan, Alan
• Is Part Of: Analytical methods. Volume 12:Issue 21(2020)
• Page Start: 2655
• Page End: 2660
• Publication Date: 2020-05-27
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/d0ay00194e
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : Bovine antibodies cannot pass the placental barrier and must be absorbed from colostrum postpartum. Monitoring of IgG absorption in calves is critical in the first 12 hours of life. Novel sensors are presented to measure this transfer process on-farm. Abstract : Bovine antibodies, such as immunoglobulin G (IgG), cannot pass the placental barrier and as such are not transferred from the mother to the foetus, in utero . Instead a calf must absorb antibodies following ingestion of colostrum postpartum. Failure of Passive Transfer (FPT) is a condition that predisposes calves to development of disease and increases the risk of mortality. Thus, continuous early monitoring of IgG absorption in a calf, within the first 6 to 12 hours of life, is imperative to allow faster treatment and prevent FPT. In this paper, we present the development of a label-free impedimetric immunosensor device for bovine IgG in serum and demonstrate its suitability to determine early FPT in new-born calves. The developed sensors were challenged to discriminate between new born calf sera, both pre- and post-colostrum feeding, and demonstrated efficient detection of IgG in under 15 minutes. Such a device could enable rapid determination of FPT, thereby improving calves' vitality and survival rate.