Rational design of minimum CRISPR guide RNA by site-specific Cas9–RNA conjugation. Issue 54 (8th June 2020)

• Record Type: Journal Article
• Authors: Ling, Xinyu; Gao, Xiaoqin; Chang, Liying; Chen, Heqi; Shi, Xiaomeng; Liu, Tao
• Is Part Of: Chemical communications. Volume 56:Issue 54(2020)
• Page Start: 7515
• Page End: 7518
• Publication Date: 2020-06-08
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d0cc01432j
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Site-specific Cas9–crRNA conjugation reduces the synthetic burden of customized guide RNA. Abstract : The CRISPR–Cas9 system enables facile and efficient genome engineering in living cells and organisms. We report a Cas9–RNA conjugation strategy to afford minimal crRNA containing only the guide sequence for the target gene, which may simplify and reduce the cost for large-scale and high-throughput crRNA synthesis and lead to new insights into the design of CRISPR family complexes for diverse purposes.