Nuclear translocation of silver ions and hepatocyte nuclear receptor impairment upon exposure to silver nanoparticles. Issue 5 (14th April 2020)

• Record Type: Journal Article
• Authors: Tardillo Suárez, Vanessa; Karepina, Elizaveta; Chevallet, Mireille; Gallet, Benoit; Cottet-Rousselle, Cécile; Charbonnier, Peggy; Moriscot, Christine; Michaud-Soret, Isabelle; Bal, Wojciech; Fuchs, Alexandra; Tucoulou, Rémi; Jouneau, Pierre-Henri; Veronesi, Giulia; Deniaud, Aurélien
• Is Part Of: Environmental science. Volume 7:Issue 5(2020)
• Page Start: 1373
• Page End: 1387
• Publication Date: 2020-04-14
• Subjects: Environmental sciences -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/c9en01348b
• Languages: English
• ISSNs: 2051-8153

Abstract

Abstract : Upon exposure to non-toxic concentration of silver nanoparticles, only the ionic form of silver is found in the nucleus leading to an endocrine disruptor-like effect. Abstract : The impact on human health of long-term exposure to low doses of silver nanoparticles (AgNPs) remains understudied. Cellular studies have shown the intracellular dissolution of AgNPs within endolysosomes followed by Ag(i ) binding to biomolecular thiolate-containing molecules. However, the precise subcellular distribution of Ag(i ) and the nature of the disrupted physiological pathways remained unknown. Novel imaging approaches enabled us to visualize the trafficking of AgNP-containing lysosomes towards a perinuclear location and a nuclear transfer of Ag(i ) species with accumulation in the nucleoli. These Ag(i ) species impaired nuclear receptor activity, disrupting critical mechanisms of liver physiology in low dose exposure scenarios, thus justifying further research into defining a framework for the safe use of AgNPs.