Microscope laser assisted photooxidative activation of bioorthogonal ClickOx probes. Issue 40 (14th April 2020)

• Record Type: Journal Article
• Authors: Kormos, Attila; Kern, Dóra; Egyed, Alexandra; Söveges, Bianka; Németh, Krisztina; Kele, Péter
• Is Part Of: Chemical communications. Volume 56:Issue 40(2020)
• Page Start: 5425
• Page End: 5428
• Publication Date: 2020-04-14
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d0cc01512a
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A photoactivatable fluorogenic tetrazine-rhodaphenothiazine probe was synthesized and studied in light-assisted, bioorthogonal labeling schemes. Abstract : A photoactivatable fluorogenic tetrazine-rhodaphenothiazine probe was synthesized and studied in light-assisted, bioorthogonal labeling schemes. Experimental results revealed that the bioorthogonally conjugated probe efficiently sensitizes 1 O2 generation upon illumination with green or orange light and undergoes self-oxidation leading to an intensely fluorescent sulfoxide product. An added value of the present probe is that it is also suitable for STED super-resolution microscopy using a 660 nm depletion laser.