An injectable BMSC-laden enzyme-catalyzed crosslinking collagen-hyaluronic acid hydrogel for cartilage repair and regeneration. Issue 19 (9th April 2020)

• Record Type: Journal Article
• Authors: Zhang, Yajie; Cao, Yi; Zhao, Hongbo; Zhang, Liwei; Ni, Tianyu; Liu, Yuanshan; An, Zhen; Liu, Min; Pei, Renjun
• Is Part Of: Journal of materials chemistry. Volume 8:Issue 19(2020)
• Page Start: 4237
• Page End: 4244
• Publication Date: 2020-04-09
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/d0tb00291g
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : An injectable BMSC-laden collagen-hyaluronic acid hydrogel crosslinked by an enzyme-catalyzed reaction was generated for cartilage repair. Abstract : Articular cartilage has limited self-healing ability due to its lack of abundant nutrients and progenitor cells. In this study, an injectable hydrogel system consisting of collagen type I-tyramine (Col-TA) and hyaluronic acid-tyramine (HA-TA) was fabricated as the bone marrow mesenchymal stem cell (BMSC)-laden hydrogel system for cartilage regeneration. Next, the physiochemical properties of this hydrogel system were well characterized and optimized, including gelation time, stiffness, water absorption and degradability. Further, the proliferation and differentiation of BMSCs within the Col-HA hydrogel were evaluated, and the ability of in vivo cartilage repair was also examined in the presence of the transforming growth factor-β1 (TGF-β1). These results illustrate that this hydrogel can offer a great microenvironment for BMSC growth and cartilage differentiation both in vitro and in vivo, and the Col-HA hydrogel can serve as an ideal hydrogel for cartilage tissue regeneration.