CRISPR/Cas-directed programmable assembly of multi-enzyme complexes. Issue 36 (2nd April 2020)
- Record Type:
- Journal Article
- Title:
- CRISPR/Cas-directed programmable assembly of multi-enzyme complexes. Issue 36 (2nd April 2020)
- Main Title:
- CRISPR/Cas-directed programmable assembly of multi-enzyme complexes
- Authors:
- Lim, Samuel
Kim, Jiwoo
Kim, Yujin
Xu, Dawei
Clark, Douglas S. - Abstract:
- Abstract : We describe a versatile CRISPR/Cas-based strategy to construct precisely organized, scaffolded multi-enzyme systems with improved productivity. Abstract : We describe a versatile CRISPR/Cas-based strategy to construct multi-enzyme complexes scaffolded on a DNA template in programmable patterns. Catalytically inactive dCas9 nuclease was used in combination with SpyCatcher–SpyTag chemistry to assemble enzymes in a highly modular fashion. Five enzymes comprising the violacein biosynthesis pathway were precisely organized in nanometer proximity; a notable increase in violacein production demonstrated the benefits of scaffolding.
- Is Part Of:
- Chemical communications. Volume 56:Issue 36(2020)
- Journal:
- Chemical communications
- Issue:
- Volume 56:Issue 36(2020)
- Issue Display:
- Volume 56, Issue 36 (2020)
- Year:
- 2020
- Volume:
- 56
- Issue:
- 36
- Issue Sort Value:
- 2020-0056-0036-0000
- Page Start:
- 4950
- Page End:
- 4953
- Publication Date:
- 2020-04-02
- Subjects:
- Chemistry -- Periodicals
540 - Journal URLs:
- http://pubs.rsc.org/en/journals/journalissues/cc ↗
http://www.rsc.org/ ↗ - DOI:
- 10.1039/d0cc01174f ↗
- Languages:
- English
- ISSNs:
- 1359-7345
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 3139.350000
British Library DSC - BLDSS-3PM
British Library STI - ELD Digital store - Ingest File:
- 13886.xml