Fabrication of a paper strip for facile and rapid detection of bovine viral diarrhea virus via signal enhancement by copper polyhedral nanoshells. Issue 50 (12th August 2020)

• Record Type: Journal Article
• Authors: Kim, Min Woo; Park, Hong-Je; Park, Chan Yeong; Kim, Ji Hong; Cho, Chae Hwan; Phan, Le Minh Tu; Park, Jong Pil; Kailasa, Suresh Kumar; Lee, Chi-Ho; Park, Tae Jung
• Is Part Of: RSC advances. Volume 10:Issue 50(2020)
• Page Start: 29759
• Page End: 29764
• Publication Date: 2020-08-12
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d0ra03677c
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : A rapid and simple affinity dot-blotting scheme via signal enhancement by copper nano-polyhedral shells on the surface of gold nanoparticles. Abstract : The detection of bovine viral diarrhea virus (BVDV), which is a pathogen inducing fatal gastrointestinal disease in cattle, is becoming a momentous issue in the livestock farm. In that, BVDV is related to inapparent infection and various diseases with high transmissibility; it has also led to considerable economic losses. In this study, a simple dot-blotting method was devised to construct a rapid screening system for BVDV. Based on the BVDV-specific bioreceptors, it was anchored on the gold nanoparticles (AuNPs) to generate the seeding sites for signaling; then the signals were amplified by adopting the overgrowth of copper nano-polyhedral shells on AuNPs. The developed detection system shows a low detection limit of 4.4 copies per mL, and even this could be distinguished with naked eyes. These results indicate that the designed nanobiosensor possesses not only high sensitivity and selectivity but also potential usage on a point-of-care testing platform for BVDV.