BSA nanoparticles as controlled release carriers for isophethalaldoxime palladacycle complex; synthesis, characterization, in vitro evaluation, cytotoxicity and release kinetics analysis. (27th February 2020)

• Record Type: Journal Article
• Authors: Karami, Kazem; Jamshidian, Nasrin; Hajiaghasi, Afsaneh; Amirghofran, Zahra
• Is Part Of: New journal of chemistry. Volume 44:Number 11(2020)
• Page Start: 4394
• Page End: 4405
• Publication Date: 2020-02-27
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/c9nj05847h
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : BSA nanoparticles were synthesized as a biodegradable carrier by the desolvation method. Abstract : A new oxime ligand named isophthalaldoxime (1 ) was synthesized by the addition of hydroxylamine hydrochloride to isophthalaldehyde. Then, a four nuclear palladacycle complex {Pd4 [( N, C )(NOHCHC6 H2 CHNOH)(μ-Cl)2 ]2 } (2 ) was prepared and fully characterized using elemental analysis, FT-IR, and NMR spectroscopy. A desolvation method was employed for the synthesis of protein nanoparticles using bovine serum albumin (BSA). Then, complex (2 ) was loaded on the prepared nanoparticles with an optimized entrapment efficiency (EE) of 97.78% and loading capacity (LC) of 22.68%. FT-IR spectroscopy and circular dichroism (CD) were used for spectral studies. Field emission scanning electron microscopy (FESEM) and dynamic light scattering (DLS) measurement was applied for structural studies on the morphology, size, and zeta potential of BSA nanoparticles (BSA-NPs) and complex-loaded nanoparticles ((2 )@BSA-NPs). The results showed the stability and acceptable size of nanoparticles prepared for the in vitro drug release experiment. Therefore, the in vitro release of (2 ) from BSA-NPs was studied using the dialysis bag method. In addition, the release mechanism was investigated by mathematical methods and the results showed that phase I and II of the release process followed Korsmeyer–Peppas and Higuchi models, respectively. Finally, the in vitro cytotoxicities of the synthesized nanoparticles, palladium complex, and complex-loaded nanoparticles were carried out against A549 human lung carcinoma and K562 human leukemia cell lines using MTT colorimetric assays.