Au–Hg/rGO with enhanced peroxidase-like activity for sensitive colorimetric determination of H2O2. Issue 6 (26th February 2020)

• Record Type: Journal Article
• Authors: Kong, Fen-Ying; Yao, Lei; Lu, Xin-Yang; Li, Heng-Ye; Wang, Zhong-Xia; Fang, Hai-Lin; Wang, Wei
• Is Part Of: Analyst. Volume 145:Issue 6(2020)
• Page Start: 2191
• Page End: 2196
• Publication Date: 2020-02-26
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/d0an00235f
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : Au–Hg/rGO is applied as a novel nanozyme to construct a sensitive sensing platform for the colorimetric determination of H2 O2 . Abstract : The Au–Hg amalgam anchored on the surface of reduced graphene oxide nanosheets (Au–Hg/rGO) has been synthesized successfully and characterized by various techniques such as transmission electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. The Au–Hg/rGO nanocomposites were found to possess excellent peroxidase-like catalytic activity and can quickly catalyze the oxidation of colorless 3, 3′, 5, 5′-tetramethylbenzidine (TMB) to blue oxTMB in the presence of H2 O2 . The obvious color change offered accurate determination of the H2 O2 concentration by recording the absorbance at 652 nm using a UV-vis spectrophotometer. The linear response range for H2 O2 was from 5 μM to 100 μM and the detection limit was 3.25 μM (S/N = 3). Furthermore, a kinetic study indicated that the catalytic behavior of Au–Hg/rGO nanocomposites followed the typical Michaelis–Menten theory and Au–Hg/rGO nanocomposites showed good affinity for H2 O2 . We envision that the simple and sensitive colorimetric detection system holds great promising applications in clinical diagnostics and food and environment monitoring.