Europium chelate-labeled lateral flow assay for rapid and multiple detection of β-lactam antibiotics by the penicillin-binding protein. Issue 28 (6th July 2020)

• Record Type: Journal Article
• Authors: Li, Xiaogang; Pan, Zhifeng; Li, Manyu; Jia, Xinmiao; Zhang, Suhan; Lin, Hang; Liu, Juntao; Ma, Liangkun
• Is Part Of: Analytical methods. Volume 12:Issue 28(2020)
• Page Start: 3645
• Page End: 3653
• Publication Date: 2020-07-06
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/d0ay01140a
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : A lateral flow assay for rapid and multiple detection of β-lactam antibiotics by the penicillin-binding protein was developed. Abstract : For the multiple and rapid detection of β-lactams, the broadly specific penicillin-binding protein (PBP) that recognizes the β-lactam structure was prepared. A chromatographic strip based on europium chelate-loaded fluorescent microspheres was assembled with a goat anti-mouse antibody (C line) and penicillin coating (T line). The penicillin coating competes with free β-lactam antibiotics to bind PBP-labeled fluorescent microspheres. The strip can theoretically detect all kinds of β-lactams, including amoxicillin, ampicillin, oxacillin, ceftazidime, lenampicillin, cefoperazone, sultamicillin, cefotaxime, ceftriaxone, sulbenicillin, piperacillin, cephalothin, flucloxacillin, and mezlocillin, in samples within 10 min. The europium chelate-labeled lateral flow assay does not cross-react with other antibiotics, including chloramphenicol, tetracycline, sulfamethazine, enrofloxacin, gentamicin or lincomycin. In short, we developed a very useful method for preliminary screening of β-lactams.