Detection of anabolic steroids via cyclodextrin-promoted fluorescence modulation. Issue 42 (1st July 2020)

• Record Type: Journal Article
• Authors: Haynes, Anna Z.; Levine, Mindy
• Is Part Of: RSC advances. Volume 10:Issue 42(2020)
• Page Start: 25108
• Page End: 25115
• Publication Date: 2020-07-01
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d0ra03485a
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Reported herein is the detection of anabolic steroids through the use of cyclodextrin-promoted interactions between the analyte and a high quantum yield fluorophore, which leads to analyte-specific changes in the fluorophore emission signal. Abstract : Reported herein is the detection of anabolic steroids through the use of cyclodextrin-promoted interactions between the analyte of interest and a high quantum yield fluorophore, which lead to measurable, analyte-specific changes in the fluorophore emission signal. By using a variety of β-cyclodextrin derivatives (unmodified β-cyclodextrin, methyl-β-cyclodextrin, and 2-hydroxypropyl-β-cyclodextrin) in combination with high quantum yield fluorophore rhodamine 6G, we detected five anabolic steroid analytes with 100% differentiation between structurally similar analytes and micromolar level limits of detection. Overall, these results show significant potential in the development of practical, fluorescence-based steroid detection devices.