The mutagenesis of a single site for enhancing or reversing the enantio- or regiopreference of cyclohexanone monooxygenases. Issue 65 (16th July 2020)

• Record Type: Journal Article
• Authors: Hu, Yujing; Xu, Weihua; Hui, Chenggong; Xu, Jian; Huang, Meilan; Lin, Xianfu; Wu, Qi
• Is Part Of: Chemical communications. Volume 56:Issue 65(2020)
• Page Start: 9356
• Page End: 9359
• Publication Date: 2020-07-16
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d0cc03721d
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Single mutation of a "second sphere" residue of CHMOs could control its enantio- and regiopreference optionally. Abstract : The mutagenesis of a "second sphere" switch residue of CHMOAcineto could control its enantio- and regiopreference. Replacing phenylalanine (F) at position 277 of CHMOAcineto into larger tryptophan (W) enabled a significant enhancement of enantio- or regioselectivity toward structurally diverse substrates, moreover, a complete reversal of enantio- or regiopreference was realized by mutating F277 into a range of smaller amino acids (A/C/D/E/G/H/I/K/L/M/N/P/Q/R/S/T/V).