A physiologically active interpenetrating collagen network that supports growth and migration of epidermal keratinocytes: zinc-polyP nanoparticles integrated into compressed collagen. Issue 27 (15th June 2020)

• Record Type: Journal Article
• Authors: Müller, Werner E. G.; Schepler, Hadrian; Tolba, Emad; Wang, Shunfeng; Ackermann, Maximilian; Muñoz-Espí, Rafael; Xiao, Shichu; Tan, Rongwei; She, Zhending; Neufurth, Meik; Schröder, Heinz C.; Wang, Xiaohong
• Is Part Of: Journal of materials chemistry. Volume 8:Issue 27(2020)
• Page Start: 5892
• Page End: 5902
• Publication Date: 2020-06-15
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/d0tb01240h
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : It is demonstrated that polyphosphate, as a component in wound healing mats together with Zn 2+, is essential for growth and migration of skin keratinocytes. Abstract : The distinguished property of the physiological polymer, inorganic polyphosphate (polyP), is to act as a bio-intelligent material which releases stimulus-dependent metabolic energy to accelerate wound healing. This characteristic is based on the bio-imitating feature of polyP to be converted, upon exposure to peptide-containing body fluids, from stable amorphous nanoparticles to a physiologically active and energy-delivering coacervate phase. This property of polyP has been utilized to fabricate a wound mat consisting of compressed collagen supplemented with amorphous polyP particles, formed from the inorganic polyanion with an over-stoichiometric ratio of zinc ions. The proliferation and the migration of human skin keratinocytes in those matrices were investigated. If the cells were embedded into the mat they respond with a significantly higher motility when zinc-polyP particles are present. Interestingly, only keratinocytes that were grown in a polyP environment developed well-structured microvilli, reflecting an increased biological activity. The data show that Zn-polyP particles incorporated into wound mats are a potent cell growth and cell migration-stimulating inorganic bio-material.